29th November 2018.
- Aptamer Group data on selective bind and elution reusability of aptamer purification columns
- Small molecule analysis webinar with Pall ForteBio BLI platform
- Our CEO Today United Kingdom award
- Interesting applications in antibiotic biosensors and ELISA antibody/aptamer pairs
Novel Solutions – Purification
Reproducibility of aptamers that bind and release under defined conditions
- Less expensive
- Gentle tailored elution
- No tags required
The immobilised aptamer captures the target protein in a complex culture harvest and then releases it in target specific elution buffer. Using Biolayer Interferometry (BLI) the regenerated surface shows repeated use of over 50+ cycles demonstrating the reproducibility of aptamers for this target and application.
Flexible Monitoring of Small Molecules in Complex Matrices with Aptamer Affinity Reagents using Bio-Layer Interferometry
December 5th, 2018 – 8am PST
The Aptamer Group is pleased to work with Pall ForteBio on this informative webinar. Aptamer Group uses Bio-Layer Interferometry (BLI) to screen and characterize the interactions of aptamers with their respective targets. The Octet® and BLItz® platforms help develop assays and give proof of concept data for the use of aptamers in other applications; like protein purification or small molecule-protein diagnostic applications.
By taking advantage of the conformational change in the aptamers upon target binding, the Octet system was implemented for high-throughput screening and characterization of aptamers against small molecule targets (~300Da). The resulting aptamers can, in turn, be used for screening samples for the presence of the target small molecule compound.
This webinar will present data to show the versatility of the BLI platforms with example data from Optimers™ for different aptamer based applications, with a focus on small molecule detection and quantification.
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In Review and News
An aptamer based assay is presented for the determination of the antibiotics oxytetracycline (OTC) and kanamycin (KAN). Magnetic beads were applied for separation, and gold nanoparticles (AuNPs) for signal amplification.
Sandwich pair of aptamers but also antbody/aptamer pairs that increased sensitivity by 3 orders of magnitude in buffer and serum.