Aptamers for siRNA delivery

15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation.  One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]

Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.

7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]

The Reusability of Aptamers for Affinity Chromatography

23rd April 2019
We have often shown that aptamers make ideal ‘capture reagents’ for use in target specific affinity chromatography. Their flexible structure makes aptamers ideally suited for the specific binding and controlled release of their target, even in the presence of complex matrixes such as cell lysates or plasma.

During the aptamer selection process, we can […]

Literature Review: Distinguishing closely-related amyloid precursors using an RNA aptamer

16 April 2019
Amyloid fibrils are misfolded proteins which have assembled to form insoluble aggregates in the form of fibers that are resistant to degradation. Amyloid formation is often associated with a disease that is specific to the proteins or localisation of the aggregate. Well known examples of amyloid diseases include Alzheimer’s disease, Diabetes type 2 […]

Literature Review: Glycan Specific Aptamers for Early Cancer Detection

9th April 2019
Changes in the glycosylation pattern of cell surface and secreted glycoproteins are common in malignant transformations and cancer progression, (1).  Prostate-specific antigen (PSA), is a glycoprotein enzyme secreted by the prostate gland that is often used for diagnosing and predicting progression of prostate cancer in men.  Recent advances in the field of glycobiology […]

Aptamers to discriminate between proteins with Post Translation Modifications

2nd April 2019
Background:

Post Translational Modifications (PTM) are biochemical variations of cetain amino acids made to a protein after biosynthesis. These modifications diversify the proteome and play a fundamental role in regulation and targeting of proteins, their interactions with other molecules, as well as their structure, activity and function. Common types of post-translational modifications include phosphorylation, […]

Literature Review: Selection, characterisation, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64

26th March 2019
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) remains one of the main causes of human death around the globe.  Mortality is high suggesting early detection and treatment would better control outbreaks and fatal outcomes.  Point of care (POC) testing for mycobacterium is challenging due to slow growth, minimal clinical symptoms of infection, and low […]

Literature Review: An Aptamer-Based Probe for Molecular Subtyping of Breast Cancer

19th March 2019
Breast cancer is a highly heterogeneous tumour conventionally classified into four molecular subtypes, i.e., Luminal A, Luminal B, HER2-enriched, and triple-negative breast cancer. In clinical practice precise molecular subtyping is a powerful approach for fast and efficient treatment and facilitates the delivery of personalised medicine approach. Immunohistochemistry (IHC) is the standard approach but the […]

Aptamer Beacons As A Simple Solution-Based Diagnostic Platform

12th March 2019
Background:

Molecular Aptamer Beacons are novel reagents capable of simple, solution-based detection and reporting of target concentration through a fluorescence-based readout.

This assay format has a number of advantages

Simple solution based assay format – no complicated incubations, wash steps etc. are required
Field-based application using a simple hand-held reader
Readily developed into multiplexed assays […]

Literature Review: Hepatocellular Carcinoma Cell Imaging

5th March 2019
Hepatocellular carcinoma (HCC) is a common malignant cancer.  Glypican-3 (GPC3), a cellular membrane proteoglycan, is highly expressed in more than 70% of HCC patients and therefore may be an ideal biomarker for in vivo HCC diagnosis and imaging. Antibody reagents have limitations such as immunogenicity, easy degradation, and high cellular cytotoxicity effect in […]