Antibiotics have been widely used in human medicine, aquaculture and agriculture to treat serious infectious diseases. However, overuse and misuse of antibiotics in some areas have contributed to the residues of antibiotic contaminants in water bodies and food products such as meat and dairy. This misuse has resulted in generation of super bacteria with tolerance […]
The current wave of COVID-19 infections around the globe has resulted from a novel coronavirus employing its spike glycoprotein to bind with the protein binding sites of the host cells. These virus particles have the notorious tendency to proteolytically generate mutated biologically active fragments involved in signal transduction pathway resulting in the proliferation and transmission […]
27th April 2020
Currently, viral infection is a serious threat for human beings. The world is facing a global pandemic associated with the COVID-19 virus, for which virulence and infectious dose data are still emerging. With almost 3,025,732 confirmed cases of COVID-19 worldwide and 209,001 deaths as of 27th April 2020 as per WHO, accurate and […]
14th April 2020
Although the world is focused on the COVID-19 virus, it is the envelope proteins that are helping companies to develop all important tests.
Benefits of Aptamers for Protein Applications
Proteins play significant roles in the physiological and pathological mechanisms of living organisms including human beings. There is an increased demand for convenient methodologies for detecting, […]
Cancer histopathology is currently the preferred method for detecting microscopic anatomical changes in tissue sections, making the discovery of cancer biomarkers critical for early diagnosis and treatment. Antibodies have been used extensively as the molecular probes; however, the production of antibodies requires a series of complicated and time-consuming processes.
There is no question that aptamers can […]
The world is currently gripped by the novel COVID-19 crisis caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is a highly pathogenic viral respiratory illness. It is an enveloped positive sense single stranded RNA virus that enters its host by binding to the ACE2 receptor (Figure 1). Two strains of the virus have […]
Osteosarcoma (OS) is a highly aggressive pediatric cancer, characterized by frequent lung metastasis and pathologic bone destruction. Vascular endothelial growth factor A (VEGFA) that is highly expressed in OS contributes to angiogenesis within the tumour microenvironment. However, it also acts as an autocrine survival factor for tumour cell themselves, making it a promising therapeutic target […]
Optimizing your PCR to avoid non-specific amplification – Aptamer mediated Hot start PCR to detect MERS-CoV
Polymerase chain reaction (PCR) is an established method to amplify specific fragments of DNA, for applications such as gene cloning, genetic fingerprinting and diagnosis of disease. Although PCR reactions have historically been set up at room temperature, this approach is highly susceptible to non-specific amplification. Such non-specific amplification often complicates data analysis and can even […]