Aptamers for siRNA delivery

15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation.  One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]

Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.

7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]

Aptamer Therapeutics and Cancer Research UK announce partnership for drug development programme

30th April 2019
Following the successful awarding of a CRUK Biotherapeutic Drug Discovery Project Award, Aptamer Therapeutics Limited, part of Aptamer Group, is pleased to announce a new collaboration with Dan Wiseman and the Oglesby Leukaemia Research Programme at the University of Manchester and Cancer Research UK. The project will explore the potential of aptamers as […]

The Reusability of Aptamers for Affinity Chromatography

23rd April 2019
We have often shown that aptamers make ideal ‘capture reagents’ for use in target specific affinity chromatography. Their flexible structure makes aptamers ideally suited for the specific binding and controlled release of their target, even in the presence of complex matrixes such as cell lysates or plasma.

During the aptamer selection process, we can […]

Literature Review: Distinguishing closely-related amyloid precursors using an RNA aptamer

16 April 2019
Amyloid fibrils are misfolded proteins which have assembled to form insoluble aggregates in the form of fibers that are resistant to degradation. Amyloid formation is often associated with a disease that is specific to the proteins or localisation of the aggregate. Well known examples of amyloid diseases include Alzheimer’s disease, Diabetes type 2 […]

Literature Review: Glycan Specific Aptamers for Early Cancer Detection

9th April 2019
Changes in the glycosylation pattern of cell surface and secreted glycoproteins are common in malignant transformations and cancer progression, (1).  Prostate-specific antigen (PSA), is a glycoprotein enzyme secreted by the prostate gland that is often used for diagnosing and predicting progression of prostate cancer in men.  Recent advances in the field of glycobiology […]

Aptamers to discriminate between proteins with Post Translation Modifications

2nd April 2019
Background:

Post Translational Modifications (PTM) are biochemical variations of cetain amino acids made to a protein after biosynthesis. These modifications diversify the proteome and play a fundamental role in regulation and targeting of proteins, their interactions with other molecules, as well as their structure, activity and function. Common types of post-translational modifications include phosphorylation, […]

Aptamer Group March 2019

31st March 2019
This month:

Novel ELISA like assay for small molecules
Moxifloxacin aptamer and intellectual property (IP) availability
Interesting applications in biologic and biosimilar analysis and cell imaging

With lots of exciting new data ongoing for multiple applications in the commercial and academic space, Aptamer Group would like to share with you information that may be […]

Literature Review: Selection, characterisation, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64

26th March 2019
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) remains one of the main causes of human death around the globe.  Mortality is high suggesting early detection and treatment would better control outbreaks and fatal outcomes.  Point of care (POC) testing for mycobacterium is challenging due to slow growth, minimal clinical symptoms of infection, and low […]

Literature Review: An Aptamer-Based Probe for Molecular Subtyping of Breast Cancer

19th March 2019
Breast cancer is a highly heterogeneous tumour conventionally classified into four molecular subtypes, i.e., Luminal A, Luminal B, HER2-enriched, and triple-negative breast cancer. In clinical practice precise molecular subtyping is a powerful approach for fast and efficient treatment and facilitates the delivery of personalised medicine approach. Immunohistochemistry (IHC) is the standard approach but the […]