25th June 2019
Small molecule targets (such as antibiotics, many drugs, toxins, food additives etc) are often perceived as difficult targets to obtain a suitable affinity ligand. They represent a challenge for traditional aptamer selection approaches which rely on immobilisation of the target molecule for efficient partitioning. Modification of the small molecule to facilitate immobilisation often […]
25th June 2019
18th June 2019
Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus and is widely used to treat various infectious diseases in humans and animals. However, like other aminoglycoside antibiotics, kanamycin exhibits comparatively narrow safety margin as high doses can cause ototoxicity, nephrotoxicity and allergic reactions.
The current methods used for analysis for detecting kanamycin levels in […]
5th June 2019
One of the key benefits to using aptamers instead of antibodies is how you store and use the reagent. Over this past 60 years the stability of oligonucleotides has been rigorously studied and well defined.
Most provides of synthetic oligonucleotides recommend storing the suspended DNA at in a neutral buffer, free from divalent metal […]
15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation. One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]
Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.
7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]
23rd April 2019
We have often shown that aptamers make ideal ‘capture reagents’ for use in target specific affinity chromatography. Their flexible structure makes aptamers ideally suited for the specific binding and controlled release of their target, even in the presence of complex matrixes such as cell lysates or plasma.
During the aptamer selection process, we can […]
9th April 2019
Changes in the glycosylation pattern of cell surface and secreted glycoproteins are common in malignant transformations and cancer progression, (1). Prostate-specific antigen (PSA), is a glycoprotein enzyme secreted by the prostate gland that is often used for diagnosing and predicting progression of prostate cancer in men. Recent advances in the field of glycobiology […]
2nd April 2019
Post Translational Modifications (PTM) are biochemical variations of cetain amino acids made to a protein after biosynthesis. These modifications diversify the proteome and play a fundamental role in regulation and targeting of proteins, their interactions with other molecules, as well as their structure, activity and function. Common types of post-translational modifications include phosphorylation, […]
Literature Review: Selection, characterisation, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64
26th March 2019
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) remains one of the main causes of human death around the globe. Mortality is high suggesting early detection and treatment would better control outbreaks and fatal outcomes. Point of care (POC) testing for mycobacterium is challenging due to slow growth, minimal clinical symptoms of infection, and low […]
19th March 2019
Breast cancer is a highly heterogeneous tumour conventionally classified into four molecular subtypes, i.e., Luminal A, Luminal B, HER2-enriched, and triple-negative breast cancer. In clinical practice precise molecular subtyping is a powerful approach for fast and efficient treatment and facilitates the delivery of personalised medicine approach. Immunohistochemistry (IHC) is the standard approach but the […]