Aptamers can be selected for use as a ‘capture reagent’ in Aptamer Mediated Affinity Chromatography (AMAC). The aptamer will discriminate between your target and the other proteins in a complete matrix (e.g. cell lysate), this makes them an ideal alternative affinity ligand for target purification.
- Processes can be tailored to include customer defined ‘loading’, ‘wash’ and ‘elution’ buffers.
- Build in protein compatible release conditions to avoid the need for harsh denaturants typically required for antibody-based purification columns.
- Increase the yield of your product by reducing losses.
Aptamers maintain function after 50+ cycles of binding, wash and release.