11th June 2019

There is a misconception that aptamer can only be used across a limited range of conditions or matrices. At Aptamer Group we start our aptamer selections with the end application in mind and generate our aptamers using conditions which closely resemble the end application. However, this shouldn’t restrict the possibilities of where an aptamer can be used.

In the past year our team have tested some of our aptamers in a broad range od matrices, to see how far we can push the function of our Moxifloxacin binding OptimerTM. As moxifloxacin is a small molecule antibiotic, we used matrices where antibiotics are likely to be tested; e.g. environmental monitoring of ground / river water, clinical monitoring in human plasma and food safety testing.

We are also able to make use of our novel, gain-of-signal aptamer based small molecule assay technology. And you can see the results below.

Figure 1: an ELISA-like assay showing the concentration dependent response to Moxifloxicin in: buffer, plasma, river water, milk, urine.

As this data shows, each of the matrices that we have tested show a concentration dependant response with similar levels of detection. The only outlier is urine with a lower response (due to the urea which is a known denaturant of aptamers). Even in this case, the response is concentration dependent and can therefore be quantified if the correct calibration standard is used.

We are confident to say that this aptamer can be used across a range of sample types commonly requested for monitoring small molecules, and this approach can also be applied and tested for the detection of other difficult targets including food/environmental contaminants, therapeutics, hormones and antibiotics.

But you don’t just have to take our word for it. This aptamer is available for you to use and we would love to see your results.

Download the data sheet here.

Or get in touch using the form below and we can send you some aptamer to test.

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