12th December 2019
Continuous improvement in imaging techniques have allowed biologists to explore with accurate methods to label cellular elements as they surpass the limit imposed by the diffraction of light. The resolution is mainly affected by the size of the affinity probes, such as antibodies which are 10-15 nm in size. Moreover, non-specificity, over labeling […]
14th November 2019
Flow cytometry has gained popularity and demand in the field of biology because of its accurate analysis and high throughput. The use of multiple lasers, affinity ligands and attached fluorophores allows simultaneous analysis of several markers expressed on thousands of cells per second (Figure 1). Some of the most widely used applications include […]
4th October 2019
Isolating a single target from a complex sample can be challenging and is often a multi step process which requires increased specificity for the target. Affinity separation is commonly used method as it involves the use of selective binding to purify proteins from a complex sample. Antibodies are commonly used as capture probes […]
19th September 2019
Purification of functional proteins is a key procedure in development and production of biologics and often relies on traditional ion exchange, hydrophobic interaction or size exclusion chromatography. Although these techniques are relatively gentle, obtaining the desired level of purity requires many sequential and sometimes repeated purification steps. Affinity separation is also frequently used […]
27th August 2019
Malaria is a serious life threatening disease, affecting a large portion of the population in developing countries. Over the last decade, there has been a continuous effort to develop efficient, stable, sensitive and low cost detection system for malaria. Currently, lateral flow based immuno-chromatographic tests are widely used for detection of malaria. However, […]
13th August 2019
Under pathophysiological conditions, transcription of proteins can lead to changes in amino acid structure known as that can either destroy their activity or impart them with new functionality. These transformations can be indicators of disease occurrence or progression and therefore point mutations on proteins can serve as useful biomarkers for disease. However, their […]
6th August 2019
Proteins can undergo post-translational modifications (PTM) of their amino acids which can lead to a change in their activity and increase their functional repertoire. The most common of these modifications include phosphorylation, glycosylation, ubiquitination, citrullination, acetylation, methylation, and hydroxylation (Figure A). PTM on proteins can therefore serve as useful biomarkers for disease. However, their […]
30th July
Blood contains a range of protein biomarkers that could be used in the early detection of disease. One important biomarker is human epidermal growth factor receptor 2 (HER2) which is overexpressed in the most common breast cancer. Various HER2 detection techniques have been reported, including FISH or IHC based assays, however, these techniques require […]
23rd July 2019
Aptamer based ‘gain of signal’ assay for small molecule detection
Detecting small molecules represents a challenge for In vitro diagnostics (IVD) due to sensitivity and specificity limits of many detection methods. Our specially designed displacement approach allows us to select aptamers that can readily be used in various IVD and point of care (POC) […]
17th July 2019
Small molecules present an ongoing challenge for antibody production and detection. Development of biosensors for the detection of small molecules is difficult due to the lack of suitable antibodies and the relatively small signals. Tools to detect and quantify them are therefore in high demand.
At Aptamer Group we select aptamers that bind to […]
