Using aptamers in different matrices

11th June 2019
There is a misconception that aptamer can only be used across a limited range of conditions or matrices. At Aptamer Group we start our aptamer selections with the end application in mind and generate our aptamers using conditions which closely resemble the end application. However, this shouldn’t restrict the possibilities of where an […]

The Stability of Aptamers

5th June 2019
One of the key benefits to using aptamers instead of antibodies is how you store and use the reagent. Over this past 60 years the stability of oligonucleotides has been rigorously studied and well defined.

Most provides of synthetic oligonucleotides recommend storing the suspended DNA at in a neutral buffer, free from divalent metal […]

Aptamers in food diagnostics

21st May 2019
Food and environmental monitoring is one of the most important aspects of dealing with recent threats to human well-being and ecosystems. Ensuring food safety is a top priority of authorities and professional players in the food supply chain.

One of the key challenges to determine the safety of food and guarantee a high level […]

Aptamers for siRNA delivery

15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation.  One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]

Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.

7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]

Aptamer Therapeutics and Cancer Research UK announce partnership for drug development programme

30th April 2019
Following the successful awarding of a CRUK Biotherapeutic Drug Discovery Project Award, Aptamer Therapeutics Limited, part of Aptamer Group, is pleased to announce a new collaboration with Dan Wiseman and the Oglesby Leukaemia Research Programme at the University of Manchester and Cancer Research UK. The project will explore the potential of aptamers as […]

The Reusability of Aptamers for Affinity Chromatography

23rd April 2019
We have often shown that aptamers make ideal ‘capture reagents’ for use in target specific affinity chromatography. Their flexible structure makes aptamers ideally suited for the specific binding and controlled release of their target, even in the presence of complex matrixes such as cell lysates or plasma.

During the aptamer selection process, we can […]

Literature Review: Distinguishing closely-related amyloid precursors using an RNA aptamer

16 April 2019
Amyloid fibrils are misfolded proteins which have assembled to form insoluble aggregates in the form of fibers that are resistant to degradation. Amyloid formation is often associated with a disease that is specific to the proteins or localisation of the aggregate. Well known examples of amyloid diseases include Alzheimer’s disease, Diabetes type 2 […]

Literature Review: Glycan Specific Aptamers for Early Cancer Detection

9th April 2019
Changes in the glycosylation pattern of cell surface and secreted glycoproteins are common in malignant transformations and cancer progression, (1).  Prostate-specific antigen (PSA), is a glycoprotein enzyme secreted by the prostate gland that is often used for diagnosing and predicting progression of prostate cancer in men.  Recent advances in the field of glycobiology […]

Aptamers to discriminate between proteins with Post Translation Modifications

2nd April 2019
Background:

Post Translational Modifications (PTM) are biochemical variations of cetain amino acids made to a protein after biosynthesis. These modifications diversify the proteome and play a fundamental role in regulation and targeting of proteins, their interactions with other molecules, as well as their structure, activity and function. Common types of post-translational modifications include phosphorylation, […]