Aptamers enhance targeted gene therapy using viral vectors

Virus mediated gene therapy has emerged as a powerful and promising option for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic, and infectious diseases and different types of cancer.

Among the viral vectors, adeno-associated virus (AAV) is particularly attractive because of its properties of low immunogenicity, high transfection efficiency and long-term transgene […]

A novel approach towards aptamer discovery based on ChIP detection method

DNA–protein interactions constitute essential biological processes such as replication, transcription and DNA repair. Mechanistic knowledge of these interactions offers insight into cell regulation, which holds the key to unlocking therapeutic targets for diseases such as cancer.

One common approach for identifying DNA sequence(s) that bind a particular protein-of-interest (POI) is based on a pull-down assay also […]

Aptamers enhance Immune Cytotoxicity against cancer cells

Aptamer based cancer immunotherapy

A promising strategy in cancer immunotherapy is the employment of a bispecific agent that can bind with both tumour markers and immunocytes for recruitment of lymphocytes to tumour sites and enhancement of anticancer immune reactions. Until recently, chimeric antigen receptor T (CAR-T) cell therapy and immune checkpoint blockade (ICB) are the remarkable […]

Aptamers used to speed up pesticide testing

19th December 2019

The contamination of the environment with pesticide residues has become one of the most serious problems of public health in the world, due to their wide application in agriculture industry to guarantee the crop yield and quality. This has necessitated the development of rapid, easy and highly sensitive approaches for the detection of […]

Aptamers provide superior staining of cellular receptors under super resolution microscopy

12th December 2019
Continuous improvement in imaging techniques have allowed biologists to explore with accurate methods to label cellular elements as they surpass the limit imposed by the diffraction of light. The resolution is mainly affected by the size of the affinity probes, such as antibodies which are 10-15 nm in size. Moreover, non-specificity, over labeling […]

Versatile probes for Flow Cytometry and FACS

14th November 2019
Flow cytometry has gained popularity and demand in the field of biology because of its accurate analysis and high throughput. The use of multiple lasers, affinity ligands and attached fluorophores allows simultaneous analysis of several markers expressed on thousands of cells per second (Figure 1). Some of the most widely used applications include […]

Purification of protein by our highly specific aptamers

4th October 2019
Isolating a single target from a complex sample can be challenging and is often a multi step process which requires increased specificity for the target. Affinity separation is commonly used method as it involves the use of selective binding to purify proteins from a complex sample. Antibodies are commonly used as  capture probes […]

Purification of functional proteins facilitated by aptamer mediated affinity chromatography (AMAC)

19th September 2019
Purification of functional proteins is a key procedure in development and production of biologics and often relies on traditional ion exchange, hydrophobic interaction or size exclusion chromatography. Although these techniques are relatively gentle, obtaining the desired level of purity requires many sequential and sometimes repeated purification steps. Affinity separation is also frequently used […]

Detection of malaria made easier with novel aptamer-based biosensor

27th August 2019
Malaria is a serious life threatening disease, affecting a large portion of the population in developing countries. Over the last decade, there has been a continuous effort to develop efficient, stable, sensitive and low cost detection system for malaria. Currently, lateral flow based immuno-chromatographic tests are widely used for detection of malaria. However, […]

Aptamers show high specificity for closely related proteins with single point mutations

13th August 2019
Under pathophysiological conditions, transcription of proteins can lead to changes in amino acid structure known as that can either destroy their activity or impart them with new functionality. These transformations can be indicators of disease occurrence or progression and therefore point mutations on proteins can serve as useful biomarkers for disease. However, their […]