The world is currently gripped by the novel COVID-19 crisis caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is a highly pathogenic viral respiratory illness. It is an enveloped positive sense single stranded RNA virus that enters its host by binding to the ACE2 receptor (Figure 1). Two strains of the virus have […]
Osteosarcoma (OS) is a highly aggressive pediatric cancer, characterized by frequent lung metastasis and pathologic bone destruction. Vascular endothelial growth factor A (VEGFA) that is highly expressed in OS contributes to angiogenesis within the tumour microenvironment. However, it also acts as an autocrine survival factor for tumour cell themselves, making it a promising therapeutic target […]
Optimizing your PCR to avoid non-specific amplification – Aptamer mediated Hot start PCR to detect MERS-CoV
Polymerase chain reaction (PCR) is an established method to amplify specific fragments of DNA, for applications such as gene cloning, genetic fingerprinting and diagnosis of disease. Although PCR reactions have historically been set up at room temperature, this approach is highly susceptible to non-specific amplification. Such non-specific amplification often complicates data analysis and can even […]
Purification of functional proteins is a key procedure in the development and production of biologics and relies on traditional ion exchange (IEX), hydrophobic interaction (HIC) or size exclusion chromatography (SEC). Although these techniques are relatively gentle, obtaining the desired level of purity requires many sequential and repeated purification steps. Affinity separation is also a frequently […]
Cystic fibrosis (CF) is one of the most lethal autosomal recessive disorder caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). It is estimated that about 60–70% of CF patients develop Pseudomonas aeruginosa chronic infection, with progressive loss of lung function, as well as increased antibiotic resistance and mortality.
Tobramycin has become an important […]
Prostate cancer is the most common cancer among men, with approximately 1.1 million new cases diagnosed per year worldwide. Most cancer-related deaths are caused by metastases in vital organs, with bones among the most frequent sites of prostate cancer metastases.
So far, various synthetic carriers have been developed to form compact nano-sized complexes carrying cancer therapeutics […]
Biotechnology companies Aptamer Group and Valitacell Ltd have joined forces to develop a next-generation screening platform for drug development.
Aptamer Group will be working alongside Valitacell to engineer the platform, in which aptamers – short sequences of DNA or RNA – are used in Valitacell’s high throughput screening processes. These screening processes can rapidly identify promising […]