19th September, 2017
An insight into working at Aptamer Group
Mateja joined Aptamer Group in August as a Selection Technician. Now she has had the chance to settle in and become established in her role, we took the opportunity to catch up with her.
Can you tell us a bit about your Ph.D.?
I have just finished the research […]
19th September, 2017
12th September, 2017
Aptamer Applications: Using Aptamers in Lateral Flow Assays
There is a growing need for easy to use, point of care tests. A technology where aptamers can be used to achieve this is in lateral flow assays. A lateral flow assay is a method of detecting the presence (or absence) of an analyte without complicated […]
5th September, 2017
At Aptamer Group we consider it vital to invest in the scientists of tomorrow. A great method for achieving this is by providing an environment where students can expand their knowledge. By having close ties with the University of York, Aptamer Group is able to support student industrial placements. One student who has […]
25th August, 2017
Starting with the end in mind
The use of aptamers in point of care diagnostic devices, for monitoring drug dose levels or for use as therapeutics, requires functionality across a wide variety of conditions.
Aptamer selection starts in conditions optimised to promote aptamer/target interaction to identify the best target-binders. Subsequently, by introducing different pressures, we […]
22nd August, 2017.
A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.
Biomarker Discovery using Aptamers
Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]
1st August, 2017.
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.
Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]
25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]
18th July, 2017
Aptamers are affinity reagents, so the mechanism of binding to a target is a key characteristic. Today we discuss how aptamers bind and outline what we do to ensure that they are fit-for-purpose.
Aptamers bind to their target through weak noncovalent forces such as; hydrogen bonding, Van der Waals and electrostatics. These occur […]
11th July, 2017
Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.
Today we outline two more strategies for utilising aptamers in biosensors.
Target Induced Displacement Mode
Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]
27th June, 2017
An application that we have proven to be successful for aptamers is Enzyme-Linked Oligonucleotide Assays (ELONA). These are similar to ELISA but utilise the benefits that aptamers offer.
ELONA use aptamers in two ways; to capture the target molecule on the plate and for use as a detection molecule. After the sample is immobilised […]