1st August 2018
Scientists from the Technical University of Denmark, led by Krishna Kant have published a new review in Biotechnology Advances including aptamer-based approaches for the rapid detection of food-borne pathogens.
Food borne diseases, caused by the consumption of foods contaminated with pathogens or their toxins, are one of the major burdens to public health, causing […]
1st August 2018
Aptamers excel as binding reagents in ELISA replacement assays for antibiotics in complex media such as plasma, milk and others
18th May 2018.
Traditionally small molecules, such as antibiotics, have been difficult targets for ELISA development. Aptamer Group has selected aptamers that bind to small molecules, previously inaccessible to other binding technologies. Utilising our state-of-the-art automation and parallel processing techniques, we can select high affinity and highly specific aptamers against small molecule targets, in solution. These […]
18th April 2018
Previously we introduced aptamers as a tool for cell specific staining. Here we have taken this technology a step further to demonstrate that aptamers can monitor disease progression in cancer.
By utilising our selection methodology, we can rapidly isolate aptamers against cells in different disease states. Through this process we have been able to identify […]
12th September, 2017
Aptamer Applications: Using Aptamers in Lateral Flow Assays
There is a growing need for easy to use, point of care tests. A technology where aptamers can be used to achieve this is in lateral flow assays. A lateral flow assay is a method of detecting the presence (or absence) of an analyte without complicated […]
22nd August, 2017.
A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.
Biomarker Discovery using Aptamers
Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]
1st August, 2017.
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.
Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]
25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]
18th July, 2017
Aptamers are affinity reagents, so the mechanism of binding to a target is a key characteristic. Today we discuss how aptamers bind and outline what we do to ensure that they are fit-for-purpose.
Aptamers bind to their target through weak noncovalent forces such as; hydrogen bonding, Van der Waals and electrostatics. These occur […]
11th July, 2017
Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.
Today we outline two more strategies for utilising aptamers in biosensors.
Target Induced Displacement Mode
Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]
27th June, 2017
An application that we have proven to be successful for aptamers is Enzyme-Linked Oligonucleotide Assays (ELONA). These are similar to ELISA but utilise the benefits that aptamers offer.
ELONA use aptamers in two ways; to capture the target molecule on the plate and for use as a detection molecule. After the sample is immobilised […]