• Aptamer, Flow Cytometry, Data, Stain, Antibody Alternative

Aptamer Application: Flow Cytometry

1st August, 2017.
Flow Cytometry
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.

Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]

  • The actions for possible aptamer based Western Blot.

Aptamer Applications: Western Blotting

25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]

Aptamer Applications: ELONA

27th June, 2017
An application that we have proven to be successful for aptamers is Enzyme-Linked Oligonucleotide Assays (ELONA). These are similar to ELISA but utilise the benefits that aptamers offer.

ELONA use aptamers in two ways; to capture the target molecule on the plate and for use as a detection molecule. After the sample is immobilised […]