Aptasensors for rapid and reliable detection of abused drugs

Drug abuse continues to be a growing global concern, causing serious health and economic implications worldwide. These drugs are known to be a strong stimulant to the central nervous system, driving up levels of dopamine, a hormone producing an additive feeling of euphoria.

Overuse or abuse of such drugs has shown to cause many adverse effects […]

Aptamer based assay for sensitive detection of antibiotics in food and water samples

Antibiotics have been widely used in human medicine, aquaculture and agriculture to treat serious infectious diseases. However, overuse and misuse of antibiotics in some areas have contributed to the residues of antibiotic contaminants in water bodies and food products such as meat and dairy. This misuse has resulted in generation of super bacteria with tolerance […]

A novel approach towards aptamer discovery based on ChIP detection method

DNA–protein interactions constitute essential biological processes such as replication, transcription and DNA repair. Mechanistic knowledge of these interactions offers insight into cell regulation, which holds the key to unlocking therapeutic targets for diseases such as cancer.

One common approach for identifying DNA sequence(s) that bind a particular protein-of-interest (POI) is based on a pull-down assay also […]

Aptamers enhance Immune Cytotoxicity against cancer cells

Aptamer based cancer immunotherapy

A promising strategy in cancer immunotherapy is the employment of a bispecific agent that can bind with both tumour markers and immunocytes for recruitment of lymphocytes to tumour sites and enhancement of anticancer immune reactions. Until recently, chimeric antigen receptor T (CAR-T) cell therapy and immune checkpoint blockade (ICB) are the remarkable […]

Aptamers provide superior staining of cellular receptors under super resolution microscopy

12th December 2019
Continuous improvement in imaging techniques have allowed biologists to explore with accurate methods to label cellular elements as they surpass the limit imposed by the diffraction of light. The resolution is mainly affected by the size of the affinity probes, such as antibodies which are 10-15 nm in size. Moreover, non-specificity, over labeling […]

Purification of protein by our highly specific aptamers

4th October 2019
Isolating a single target from a complex sample can be challenging and is often a multi step process which requires increased specificity for the target. Affinity separation is commonly used method as it involves the use of selective binding to purify proteins from a complex sample. Antibodies are commonly used as  capture probes […]

Purification of functional proteins facilitated by aptamer mediated affinity chromatography (AMAC)

19th September 2019
Purification of functional proteins is a key procedure in development and production of biologics and often relies on traditional ion exchange, hydrophobic interaction or size exclusion chromatography. Although these techniques are relatively gentle, obtaining the desired level of purity requires many sequential and sometimes repeated purification steps. Affinity separation is also frequently used […]

Aptamers show high specificity for closely related proteins with single point mutations

13th August 2019
Under pathophysiological conditions, transcription of proteins can lead to changes in amino acid structure known as that can either destroy their activity or impart them with new functionality. These transformations can be indicators of disease occurrence or progression and therefore point mutations on proteins can serve as useful biomarkers for disease. However, their […]

Aptamers to discriminate closely related proteins with post-translational modifications

6th August 2019

Proteins can undergo post-translational modifications (PTM) of their amino acids which can lead to a change in their activity and increase their functional repertoire. The most common of these modifications include phosphorylation, glycosylation, ubiquitination, citrullination, acetylation, methylation, and hydroxylation (Figure A). PTM on proteins can therefore serve as useful biomarkers for disease. However, their […]

Biomarker detection in serum becomes easier using aptamer based biosensor

30th July

Blood contains a range of protein biomarkers that could be used in the early detection of disease. One important biomarker is human epidermal growth factor receptor 2 (HER2) which is overexpressed in the most common breast cancer. Various HER2 detection techniques have been reported, including FISH or IHC based assays, however, these techniques require […]