12th September, 2017
Aptamer Applications: Using Aptamers in Lateral Flow Assays
There is a growing need for easy to use, point of care tests. A technology where aptamers can be used to achieve this is in lateral flow assays. A lateral flow assay is a method of detecting the presence (or absence) of an analyte without complicated […]
12th September, 2017
22nd August, 2017.
A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.
Biomarker Discovery using Aptamers
Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]
1st August, 2017.
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.
Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]
25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]
18th July, 2017
Aptamers are affinity reagents, so the mechanism of binding to a target is a key characteristic. Today we discuss how aptamers bind and outline what we do to ensure that they are fit-for-purpose.
Aptamers bind to their target through weak noncovalent forces such as; hydrogen bonding, Van der Waals and electrostatics. These occur […]
11th July, 2017
Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.
Today we outline two more strategies for utilising aptamers in biosensors.
Target Induced Displacement Mode
Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]
Aptamers are increasingly being used in Biosensors. Biosensors are devices that use biological molecules, especially enzymes or antibodies, or living organisms to detect the presence of an analyte. By modifying an aptamer with an appropriate reporter group, we are able to develop biosensors that measure analytes qualitatively and/or quantitatively.
Modes in which aptamers are utilised […]
27th June, 2017
An application that we have proven to be successful for aptamers is Enzyme-Linked Oligonucleotide Assays (ELONA). These are similar to ELISA but utilise the benefits that aptamers offer.
ELONA use aptamers in two ways; to capture the target molecule on the plate and for use as a detection molecule. After the sample is immobilised […]
23rd May 2017
AptaBasics: Aptamer Selection
At Aptamer Group we can select and identify novel aptamers to specific targets in as little as 12 weeks. To select an aptamer we screen our aptamer library of about 1015 molecules, isolating monoclonal aptamers with the required affinity and selectivity for the customer nominated target of interest.
The selection process begins […]
16th May 2017
AptaBasics: What is an Aptamer Library?
Last week we described what aptamers are and the fact they have a variable region. This week we will discuss the scale of the library and why this makes aptamers suitable to many applications.
To generate the largest possible aptamer library, we start with a combinational library of DNA […]