Aptamers in therapeutic drug monitoring for Cystic Fibrosis

Cystic fibrosis (CF) is one of the most lethal autosomal recessive disorder caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). It is estimated that about 60–70% of CF patients develop Pseudomonas aeruginosa chronic infection, with progressive loss of lung function, as well as increased antibiotic resistance and mortality.

Tobramycin has become an important […]

Aptamers in prostate cancer in vivo research

Prostate cancer is the most common cancer among men, with approximately 1.1 million new cases diagnosed per year worldwide. Most cancer-related deaths are caused by metastases in vital organs, with bones among the most frequent sites of prostate cancer metastases.

So far, various synthetic carriers have been developed to form compact nano-sized complexes carrying cancer therapeutics […]

Aptamers provide superior staining of cellular receptors under super resolution microscopy

12th December 2019
Continuous improvement in imaging techniques have allowed biologists to explore with accurate methods to label cellular elements as they surpass the limit imposed by the diffraction of light. The resolution is mainly affected by the size of the affinity probes, such as antibodies which are 10-15 nm in size. Moreover, non-specificity, over labeling […]

Detection of malaria made easier with novel aptamer-based biosensor

27th August 2019
Malaria is a serious life threatening disease, affecting a large portion of the population in developing countries. Over the last decade, there has been a continuous effort to develop efficient, stable, sensitive and low cost detection system for malaria. Currently, lateral flow based immuno-chromatographic tests are widely used for detection of malaria. However, […]

Novel gain of signal approach for small molecule detection

17th July 2019
Small molecules present an ongoing challenge for antibody production and detection. Development of biosensors for the detection of small molecules is difficult due to the lack of suitable antibodies and the relatively small signals. Tools to detect and quantify them are therefore in high demand.

At Aptamer Group we select aptamers that bind to […]

Small molecule detection made easier using Fluorescence Anisotropy technique

9th July 2019
Traditionally, immuno-fluorescence polarization-based assays are used to detect small molecules by using antibodies and fluorescently labelled small-molecules. However, the assays do meet limitations such as complex antibody preparation, cost, and most importantly stability. Also the preparation of fluorescently labelled small molecules is challenging. The use of aptamers overcomes the limitations and brings new […]

  • An Aptamer Group robot performing aptamer selection.

Our Small Moleule Approach

25th June 2019
Small molecule targets (such as antibiotics, many drugs, toxins, food additives etc) are often perceived as difficult targets to obtain a suitable affinity ligand. They represent a challenge for traditional aptamer selection approaches which rely on immobilisation of the target molecule for efficient partitioning. Modification of the small molecule to facilitate immobilisation often […]

Literature review: Rapid detection of Kanamycin antibiotic in complex food matrix

18th June 2019
Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus and is widely used to treat various infectious diseases in humans and animals. However, like other aminoglycoside antibiotics, kanamycin exhibits comparatively narrow safety margin as high doses can cause ototoxicity, nephrotoxicity and allergic reactions.

The current methods used for analysis for detecting kanamycin levels in […]

Aptamers for siRNA delivery

15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation.  One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]

Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.

7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]