12th December 2019
Continuous improvement in imaging techniques have allowed biologists to explore with accurate methods to label cellular elements as they surpass the limit imposed by the diffraction of light. The resolution is mainly affected by the size of the affinity probes, such as antibodies which are 10-15 nm in size. Moreover, non-specificity, over labeling […]
12th December 2019
27th August 2019
Malaria is a serious life threatening disease, affecting a large portion of the population in developing countries. Over the last decade, there has been a continuous effort to develop efficient, stable, sensitive and low cost detection system for malaria. Currently, lateral flow based immuno-chromatographic tests are widely used for detection of malaria. However, […]
17th July 2019
Small molecules present an ongoing challenge for antibody production and detection. Development of biosensors for the detection of small molecules is difficult due to the lack of suitable antibodies and the relatively small signals. Tools to detect and quantify them are therefore in high demand.
At Aptamer Group we select aptamers that bind to […]
9th July 2019
Traditionally, immuno-fluorescence polarization-based assays are used to detect small molecules by using antibodies and fluorescently labelled small-molecules. However, the assays do meet limitations such as complex antibody preparation, cost, and most importantly stability. Also the preparation of fluorescently labelled small molecules is challenging. The use of aptamers overcomes the limitations and brings new […]
25th June 2019
Small molecule targets (such as antibiotics, many drugs, toxins, food additives etc) are often perceived as difficult targets to obtain a suitable affinity ligand. They represent a challenge for traditional aptamer selection approaches which rely on immobilisation of the target molecule for efficient partitioning. Modification of the small molecule to facilitate immobilisation often […]
18th June 2019
Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus and is widely used to treat various infectious diseases in humans and animals. However, like other aminoglycoside antibiotics, kanamycin exhibits comparatively narrow safety margin as high doses can cause ototoxicity, nephrotoxicity and allergic reactions.
The current methods used for analysis for detecting kanamycin levels in […]
15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation. One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]
Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.
7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]
30th April 2019
Following the successful awarding of a CRUK Biotherapeutic Drug Discovery Project Award, Aptamer Therapeutics Limited, part of Aptamer Group, is pleased to announce a new collaboration with Dan Wiseman and the Oglesby Leukaemia Research Programme at the University of Manchester and Cancer Research UK. The project will explore the potential of aptamers as […]
23rd April 2019
We have often shown that aptamers make ideal ‘capture reagents’ for use in target specific affinity chromatography. Their flexible structure makes aptamers ideally suited for the specific binding and controlled release of their target, even in the presence of complex matrixes such as cell lysates or plasma.
During the aptamer selection process, we can […]