Cell specific staining

Cell specific staining in Confocal Imaging
Over the next few weeks we are going to look at examples of where we have developed aptamers for cell imaging.

One of the most common methods for understanding the structure and function of biological systems is through confocal microscopy. Cell imaging technologies have been advancing at a rapid pace, however […]

  • Comparing healthy to disease state cells using aptamers

Aptamer Application: Biomarker Discovery

22nd August, 2017.
Biomarker Discovery

A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.

Biomarker Discovery using Aptamers

Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]

  • Aptamer, Flow Cytometry, Data, Stain, Antibody Alternative

Aptamer Application: Flow Cytometry

1st August, 2017.
Flow Cytometry
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.

Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]

  • The actions for possible aptamer based Western Blot.

Aptamer Applications: Western Blotting

25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]

  • Aptamer affinity, aptamer selection

AptaBasics: Aptamer Affinity

18th July, 2017
Aptamer Affinity
Aptamers are affinity reagents, so the mechanism of binding to a target is a key characteristic. Today we discuss how aptamers bind and outline what we do to ensure that they are fit-for-purpose.

Aptamers bind to their target through weak noncovalent forces such as; hydrogen bonding, Van der Waals and electrostatics. These occur […]

  • A graph showing the change in signal with the introduction of a target in a TID signal off biosensor

Aptamer Applications: Biosensors part 2

11th July, 2017

Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.

Today we outline two more strategies for utilising aptamers in biosensors.

Target Induced Displacement Mode

Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]

Aptamer Applications: Biosensors part 1

4th July

Aptamers are increasingly being used in Biosensors. Biosensors are devices that use biological molecules, especially enzymes or antibodies, or living organisms to detect the presence of an analyte. By modifying an aptamer with an appropriate reporter group, we are able to develop biosensors that measure analytes qualitatively and/or quantitatively.

Modes in which aptamers are utilised […]

  • Library vs log scale of molecules present.

What is an Aptamer Library?

16th May 2017
AptaBasics: What is an Aptamer Library?
Last week we described what aptamers are and the fact they have a variable region. This week we will discuss the scale of the library and why this makes aptamers suitable to many applications.

To generate the largest possible aptamer library, we start with a combinational library of DNA […]

  • 2 aptamers binding to small molecule targets and one aptamer binding to a large molecule target

What is an aptamer?

10th May 2017
AptaBasics:  ‘What is an Aptamer’?

An aptamer is a sequence of single strand nucleic acid (DNA or RNA) with a variable region of about 40 nucleotide bases. This variable sequence confers each aptamer with a unique 3 dimensional structure and potential ligand binding capability.  These factors make aptamers high affinity and specific binding molecules, […]