25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]
25th July, 2017
18th July, 2017
Aptamers are affinity reagents, so the mechanism of binding to a target is a key characteristic. Today we discuss how aptamers bind and outline what we do to ensure that they are fit-for-purpose.
Aptamers bind to their target through weak noncovalent forces such as; hydrogen bonding, Van der Waals and electrostatics. These occur […]
11th July, 2017
Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.
Today we outline two more strategies for utilising aptamers in biosensors.
Target Induced Displacement Mode
Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]
Aptamers are increasingly being used in Biosensors. Biosensors are devices that use biological molecules, especially enzymes or antibodies, or living organisms to detect the presence of an analyte. By modifying an aptamer with an appropriate reporter group, we are able to develop biosensors that measure analytes qualitatively and/or quantitatively.
Modes in which aptamers are utilised […]
16th May 2017
AptaBasics: What is an Aptamer Library?
Last week we described what aptamers are and the fact they have a variable region. This week we will discuss the scale of the library and why this makes aptamers suitable to many applications.
To generate the largest possible aptamer library, we start with a combinational library of DNA […]
10th May 2017
AptaBasics: ‘What is an Aptamer’?
An aptamer is a sequence of single strand nucleic acid (DNA or RNA) with a variable region of about 40 nucleotide bases. This variable sequence confers each aptamer with a unique 3 dimensional structure and potential ligand binding capability. These factors make aptamers high affinity and specific binding molecules, […]