15th May 2019
siRNA’s are functional double stranded RNA molecules which can be used to control gene expression by targeting a messenger RNA and promoting its degradation. One of the challenges in the therapeutic application of siRNA’s is targeting and delivery of the siRNA, to the cell / tissue of interest. Various adjuvants have been tested […]
15th May 2019
Literature Review: Distinguishing glycosylation patterns in proteins for the early detection of cancer.
7th May 2019
Glycosylation is a post-translational modification where a carbohydrate is added to a protein. Glycosylation of proteins serves a number of functions in a cell, including: improve protein folding, forming lipid anchors and cell-to-cell adhesion. While glycosylation of a protein can be vital for its function, any changes in the position or number of […]
4th May 2018
Development of biosensors for the detection of small molecules is notoriously difficult due to the lack of suitable antibodies and the relatively small signals, which result upon small molecule binding to them. At Aptamer Group we select aptamers that bind to small molecules with high specificity and undergo a substantial conformational change that gives […]
18th April 2018
Previously we introduced aptamers as a tool for cell specific staining. Here we have taken this technology a step further to demonstrate that aptamers can monitor disease progression in cancer.
By utilising our selection methodology, we can rapidly isolate aptamers against cells in different disease states. Through this process we have been able to identify […]
Cell specific staining in Confocal Imaging
Over the next few weeks we are going to look at examples of where we have developed aptamers for cell imaging.
One of the most common methods for understanding the structure and function of biological systems is through confocal microscopy. Cell imaging technologies have been advancing at a rapid pace, however […]
25th August, 2017
Starting with the end in mind
The use of aptamers in point of care diagnostic devices, for monitoring drug dose levels or for use as therapeutics, requires functionality across a wide variety of conditions.
Aptamer selection starts in conditions optimised to promote aptamer/target interaction to identify the best target-binders. Subsequently, by introducing different pressures, we […]
22nd August, 2017.
A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.
Biomarker Discovery using Aptamers
Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]
1st August, 2017.
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.
Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]
26 August 2016
Aptamers have emerged as an exciting and promising new means of treating neurological disease, with the potential to fundamentally change the way we approach CNS-targeted therapeutics. With the propensity to cross the blood–brain barrier (BBB), Aptamers can target specific cell or signalling systems, respond to endogenous stimuli, or act as vehicles for gene […]
08 June 2016
The immune system plays a vital role in suppressing and eliminating cancer. Immunotherapeutics targeting tumour cell biology has become a popular field of biomedicine and there remains a great deal of excitement surrounding this. The emergence of antibody therapies against molecular regulators including CTLA-4 and PD-1 have shown positive results in clinical trials […]