Aptamer based delivery of CRISPR/Cas9 for cancer gene therapy without the need for electroporation

CRISPR/Cas9 therapeutics have been in vitro techniques historically. This review shows the promise of potential in vivo aptamer based delivery of CRISPR/Cas9 therapeutics.

Precise editing of DNA sequences in the human genome can correct mutations or introduce novel genetic functionality, thus providing promising solutions to various diseases. Amongst the various diverse gene editing technology to […]

Aptamers and their applications in RNAi technology

The RNA interference (RNAi) era, started by Fire and Mello in 1998 completely changed the vision of research involving cell control and therapeutic intervention. RNAi machinery starts from a long dsRNA and pre-microRNA (pre-miRNA) that is cleaved by the ‘Dicer’ enzyme in a short dsRNA fragments called small interfering RNA (siRNA) and miRNA, respectively. This, […]

Aptamers enhance targeted gene therapy using viral vectors

Virus mediated gene therapy has emerged as a powerful and promising option for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic, and infectious diseases and different types of cancer.

Among the viral vectors, adeno-associated virus (AAV) is particularly attractive because of its properties of low immunogenicity, high transfection efficiency and long-term transgene […]