Aptamer based biosensors for rapid and sensitive detection of toxins

#aptamers #biosensors #toxins #aptasensors #sensitivedetection #rapiddetection #antibodyalternative #highselectivity #microbialdetection #foodsafety #smallmolecules #aptamergroup #COVID19research #aptamerscience

Aptamers to discriminate between proteins with Post Translation Modifications

2nd April 2019
Background:

Post Translational Modifications (PTM) are biochemical variations of cetain amino acids made to a protein after biosynthesis. These modifications diversify the proteome and play a fundamental role in regulation and targeting of proteins, their interactions with other molecules, as well as their structure, activity and function. Common types of post-translational modifications include phosphorylation, […]

Aptamer Stability

29th August 2018
At Aptamer Group, every custom aptamer project begins with a feasibility study. The target and assay matrix are tested for compatibility and stability with the aptamer library, under a variety of conditions. At this early stage it is important to understand the optimal strategy for the selection, address any potential issues and […]

Aptamer-based Lab on chip for the rapid detection of food-borne pathogens

1st August 2018
Scientists from the Technical University of Denmark, led by  Krishna  Kant have published a new review in Biotechnology Advances including  aptamer-based approaches  for the rapid detection of food-borne pathogens.
Food borne diseases, caused by the consumption of foods contaminated with pathogens or their toxins, are one of the major burdens to public health, causing […]

Aptamers excel as binding reagents in ELISA replacement assays for antibiotics in complex media such as plasma, milk and others

18th May 2018.

Traditionally small molecules, such as antibiotics, have been difficult targets for ELISA development. Aptamer Group has selected aptamers that bind to small molecules, previously inaccessible to other binding technologies. Utilising our state-of-the-art automation and parallel processing techniques, we can select high affinity and highly specific aptamers against small molecule targets, in solution. These […]

Cell Imaging – Aptamers can monitor the different stages of cancer

18th April 2018
Previously we introduced aptamers as a tool for cell specific staining. Here we have taken this technology a step further to demonstrate that aptamers can monitor disease progression in cancer.

By utilising our selection methodology, we can rapidly isolate aptamers against cells in different disease states. Through this process we have been able to identify […]

Cell specific staining

Cell specific staining in Confocal Imaging
Over the next few weeks we are going to look at examples of where we have developed aptamers for cell imaging.

One of the most common methods for understanding the structure and function of biological systems is through confocal microscopy. Cell imaging technologies have been advancing at a rapid pace, however […]

  • A diagram of the lateral flow mechanism

Lateral Flow Assays

12th September, 2017
Aptamer Applications: Using Aptamers in Lateral Flow Assays
There is a growing need for easy to use, point of care tests. A technology where aptamers can be used to achieve this is in lateral flow assays. A lateral flow assay is a method of detecting the presence (or absence) of an analyte without complicated […]

  • Comparing healthy to disease state cells using aptamers

Aptamer Application: Biomarker Discovery

22nd August, 2017.
Biomarker Discovery

A biomarker is a measurable indicator showing the presence or severity of a disease state. Using biomarkers to differentiate between healthy and diseased cells can enable the development of specific diagnostics, research tools and targeted therapeutics.

Biomarker Discovery using Aptamers

Traditional biomarker discovery is a long process and relies on multiple comparisons using various […]

  • Aptamer, Flow Cytometry, Data, Stain, Antibody Alternative

Aptamer Application: Flow Cytometry

1st August, 2017.
Flow Cytometry
Flow Cytometry is a technique used to identify biomarkers on cell surfaces and intracellular molecules. Most flow cytometers use fluorescently labelled antibodies to differentiate between cells expressing different markers.

Initially the cell sample is suspended, stained with a fluorescent label and washed to remove any non-specific binding. The suspension is introduced into the […]