• The actions for possible aptamer based Western Blot.

Aptamer Applications: Western Blotting

25th July, 2017
AptaBlot – increasing the utility of Western Blotting
Western Blotting is a powerful technique used to detect and identify proteins. Antibodies have traditionally been used as the detection molecule, but aptamers are now emerging to complement or address shortfalls of existing reagents.
Basis of the Western Blot
The first step of a Western blot uses electrophoresis […]

  • A graph showing the change in signal with the introduction of a target in a TID signal off biosensor

Aptamer Applications: Biosensors part 2

11th July, 2017

Last week we began to discuss how aptamers can be used in biosensors. If you missed this, you can read it here.

Today we outline two more strategies for utilising aptamers in biosensors.

Target Induced Displacement Mode

Target Induced Displacement (TID) mode uses an aptamer anchored via a complementary oligo, localising the target binding aptamer. When […]

  • 2 aptamers binding to small molecule targets and one aptamer binding to a large molecule target

What is an aptamer?

10th May 2017
AptaBasics:  ‘What is an Aptamer’?

An aptamer is a sequence of single strand nucleic acid (DNA or RNA) with a variable region of about 40 nucleotide bases. This variable sequence confers each aptamer with a unique 3 dimensional structure and potential ligand binding capability.  These factors make aptamers high affinity and specific binding molecules, […]