Why Our Aptamers?
Why Our Aptamers?
After 5 years of process development our proprietary, automated, processes enable us to identify functional aptamers with high success rates, high affinity and highly specific in a short time frame, approximately 8 weeks. As standard, we build future requirements of performance and functionality into our selection process and develop bespoke parametrised processes, unique to each customer’s needs resulting in a bespoke solution as opposed to a rigid service.
- Our automated processes follow a design of experiments (DOE) approach and start with full characterisation of the targets and the constituent elements of the end application matrix. This allows us to identify the best conditions for successful aptamer selection.
- We identify suitable conditions to promote interactions with the target(s) and then demonstrate binding between aptamer library and target(s). This initial stage enables us to make sure that it is possible for the aptamer library to interact with the target. In the unlikely event that we are unable to promote binding to the target, the project stops at the customer incurs no further costs.
- In every selection round we trial a multitude of different selection parameters using high throughput robotics to identify the best ‘route’ through the selection. We build in functionality screening at each stage to ensure that the aptamers are not just binders, but also functional in the end assay.
- We give our customers binding data which demonstrates the aptamers have suitable affinity and are specific for the target. In projects where functionality is required, this is built into the approach and is also reported.
- At multiple points during each project cycle we demonstrate that our aptamers work giving us a higher chance of success and meeting project specifications.
- We show at every possible stage that our aptamer libraries, pools and clones bind to the targets and perform functionally. Furthermore, as standard, we identify the minimal functional aptamer fragment – we call the output of this process an OptimerTM (an ‘optimum’ aptamer).